Empore™ StageTips: Precise peptide prep for proteomics
Empore™ StageTips: Precise peptide prep for proteomics
Precise sample preparation starts with the right tip. Learn how Empore™ StageTips — with 30+ sorbent chemistries and proven membrane technology — simplify peptide prep in routine proteomics workflows.
Product spotlight
Empore™ StageTips: Precise peptide prep for proteomics
Pre-packed SPE micropipette tips built on Empore™ membrane technology — trusted by proteomics researchers for over 20 years. Available in more than 30 sorbent chemistries for peptide desalting, fractionation, and integrated workflows.
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Proteomics Optimized
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Pre-Packed & Ready
✓
30+ Sorbent Chemistries
What are StageTips?
StageTips are pre-packed solid phase extraction (SPE) tips that combine ease of use with the proven performance of Empore™ membrane technology. Each tip is machine-pressed in an ISO-9001 clean room using automated precision tooling — eliminating the variability of manual packing and delivering consistent sorbent loading across every tip in every lot.
Available in 10 µL and 200 µL formats, StageTips cover the full range from ultra-sensitive single-cell proteomics to standard routine workflows. Up to 6 sorbent layers can be stacked in a single tip, enabling integrated desalting and fractionation in one step.
Technical Specifications
10, 100 & 200 µL
Tip Volumes
Up to 6
Max Sorbent Layers
Polypropylene
Tip Material
All Organic
Solvent Compatibility
r = 0.99
Tip-to-Tip Reproducibility
~3 Years
Shelf Life (Room Temp)
96 / 384 tips
Pack Size
Up to 30
Sorbent Chemistries
Find the right StageTip
Choose your application first, then select the sorbent chemistry that best fits your sample type and downstream workflow.
Peptide desalting
C18-HD
General peptide desalting — the most widely used sorbent. Recommended for acidic, neutral, and basic pH.
Desalting10 / 200 µL
C8-HD
Moderately hydrophobic peptides. Alternative to C18 for shorter or less hydrophobic peptides.
Desalting10 / 200 µL
SDB-XC
Broad polarity peptide cleanup. Styrenedivinylbenzene polymer for mixed polarity analytes.
Desalting10 / 200 µL
SDB-RPS
Acidic peptides, mixed-mode retention. Combines nonpolar and cation exchange for broad coverage.
Desalting10 / 200 µL
HLB-SD
Hydrophilic-lipophilic balance. Broad selectivity for both polar and nonpolar peptides.
Desalting10 / 200 µL
Peptide fractionation
SDB-RPS
High-pH reverse phase fractionation. Orthogonal to low-pH LC-MS — 53% more peptides in our HeLa benchmark.
Fractionation10 / 200 µL
SCX
Strong cation exchange fractionation. Stepwise elution with increasing salt concentration.
Fractionation10 / 200 µL
SAX
Strong anion exchange fractionation. Stepwise elution with decreasing pH or increasing salt.
Fractionation10 / 200 µL
C18-HD
High-pH reverse phase fractionation. Orthogonal to standard low-pH LC-MS.
Fractionation10 / 200 µL
MCX-SD
Mixed-mode cation exchange fractionation. Selective retention and stepwise elution for basic peptides in complex matrices.
Fractionation10 / 200 µL
Integrated workflow
C18 / SCX
Combined desalting and cation exchange in a two-sorbent configuration for integrated cleanup and fractionation.
Integrated200 µL
C18 / SCX / C18
Three-sorbent sandwich for advanced fractionation and maximum workflow flexibility.
Integrated10 / 200 µL
High-Throughput
Empore 384 StageTips
Pre-packed StageTips in a 384-well tray format for high-throughput proteomics labs. Available in more than 30 sorbents.
384
Tips per Tray (24 × 16)
× 2
Trays per pack
100 µL
Tip Volume
2-layer
Available in 30+ sorbents
✓ Lower per-tip cost vs. standard 96-packs
✓ Available in all Empore sorbent chemistries (30+ options)
✓ Compatible with multichannel pipettes and automation
✓ Same Empore™ membrane quality and reproducibility
Why labs choose Empore StageTips
01
Pre-packed by automated precision tooling
Every tip is machine-pressed in our ISO-9001 clean room. No manual packing, no variability — open the pack and start your experiment.
02
Excellent lot-to-lot reproducibility
QA/QC testing across multiple lots shows no significant variation (p > 0.05) in protein identification and PSM counts.
03
30+ sorbent chemistries, up to 6 layers
C18, C8, SDB-XC, SDB-RPS, SAX, SCX, HLB, and pre-configured multi-layer combinations for desalting and fractionation in a single tip.
04
Automatable and high-throughput
Use rack adaptors to process StageTips by centrifugation — hands-free, scalable, and compatible with multichannel pipettes.
C18 desalting in 5 steps
Complete peptide cleanup in under 30 minutes with our validated 5-step protocol — from sample preparation to MS-ready peptides. All steps at 1,000–1,500 × g.
1
Condition
Filter 50–100 µL methanol. Keep membrane wet.
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2
Equilibrate
Filter 50–100 µL of 0.5% TFA. Keep membrane wet.
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3
Load Sample
Pipette sample into tip. Max 400 µL, 40 µg peptide per C18-HD tip.
The 10 µL format is built for ultra-sensitive, micro-scale proteomics — including single-cell applications with sample volumes as low as 0.1 µL. Available in C18-HD and SDB-RPS. The 200 µL format covers standard workflows up to 200 µL and is available across all sorbent chemistries with multi-layer configurations.
How many sorbent layers do you need?
2-layer tips handle most desalting tasks. For higher binding capacity — when processing more than 20 µg peptide or working with dilute samples — more layers help. C18-HD is available in 2, 3, 4, and 6 layers; SDB-XC and SDB-RPS in 2, 4, and 6 layers.
About the Empore 384 format
384 StageTips per tray, two trays per pack, 100 µL tip volume. Available across all 30+ Empore sorbent chemistries. Designed for high-throughput labs processing large sample batches — with lower per-tip costs and full compatibility with multichannel pipettes and automation.
Published with the kind support and permission of CDS Analytical. All product information, protocols, and data refer to Empore™ StageTips by CDS Analytical.